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中华姬鼠与大林姬鼠的同工酶差异
引用本文:傅必谦 李举怀. 中华姬鼠与大林姬鼠的同工酶差异[J]. 兽类学报, 1998, 18(4): 304-310
作者姓名:傅必谦 李举怀
作者单位:首都师范大学生物系,北京市农林科学院
摘    要:
中华姬鼠(Apodemusdraco)和大林姬鼠(Apodemuspeninsulae)是形态学上十分相似的两种鼠类。为了对两种姬鼠的分类提供生物化学方面的依据,采用聚丙烯酰胺凝胶等电聚焦电泳方法比较和分析了两种姬鼠的LDH同工酶、EST同工酶和SOD同工酶的差异。结果表明,两种姬鼠的LDH同工酶酶谱基本相似,而EST同工酶和SOD同工酶酶谱则存在明显的种间差异。根据EST同工酶A2带的有无和SOD同工酶主带等电点的差别,能将两种姬鼠很容易区分开来。

关 键 词:中华姬鼠;大林姬鼠;LDH同工酶;EST同工酶;SOD同工酶;生化分类

INTERSPECIFIC DIFFERENCES OF ISOZYME SYSTEMS BETWEEN APODEMUS DRACO AND APODEMUS PENINSULAE
FU Biqian CHEN Wei GAO Wu ZHANG Kexin YUAN Hong RUI Chaosheng. INTERSPECIFIC DIFFERENCES OF ISOZYME SYSTEMS BETWEEN APODEMUS DRACO AND APODEMUS PENINSULAE[J]. Acta Theriologica Sinica, 1998, 18(4): 304-310
Authors:FU Biqian CHEN Wei GAO Wu ZHANG Kexin YUAN Hong RUI Chaosheng
Abstract:
Apodemus draco and A.peninsulae are the two species quite similar in morphological characters. In order to explore some diagnostic means of the biochemical taxonomy of the two species, LDH, EST and SOD isozymes were isolated from leg muscles,breast muscles,hearts,kidneys,livers,spleens,lungs and brains of A. draco and A.peninsulae and were analyzed by isoelectric focusing with PAGE. Distinct interspecific differences were found in EST and SOD electrophoregrams,but not found in LDH electrophoregrams.It was suggested that A.draco and A.peninsulae could be unequivocally distinguished by the presence or absence of A 2 band in EST isozymes or by the variations of isoelectric point(pI)of main bands in SOD isozymes.
Keywords:Apodemus draco  Apodemus peninsulae  EST isozymes  LDH isozymes  SOD isozymes  Biochemical taxonomy
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