中华姬鼠与大林姬鼠的同工酶差异

中华姬鼠（Ａｐｏｄｅｍｕｓｄｒａｃｏ）和大林姬鼠（Ａｐｏｄｅｍｕｓｐｅｎｉｎｓｕｌａｅ）是形态学上十分相似的两种鼠类。为了对两种姬鼠的分类提供生物化学方面的依据，采用聚丙烯酰胺凝胶等电聚焦电泳方法比较和分析了两种姬鼠的ＬＤＨ同工酶、ＥＳＴ同工酶和ＳＯＤ同工酶的差异。结果表明，两种姬鼠的ＬＤＨ同工酶酶谱基本相似，而ＥＳＴ同工酶和ＳＯＤ同工酶酶谱则存在明显的种间差异。根据ＥＳＴ同工酶Ａ２带的有无和ＳＯＤ同工酶主带等电点的差别，能将两种姬鼠很容易区分开来。

INTERSPECIFIC DIFFERENCES OF ISOZYME SYSTEMS BETWEEN APODEMUS DRACO AND APODEMUS PENINSULAE

Apodemus draco and A.peninsulae are the two species quite similar in morphological characters. In order to explore some diagnostic means of the biochemical taxonomy of the two species, LDH, EST and SOD isozymes were isolated from leg muscles,breast muscles,hearts,kidneys,livers,spleens,lungs and brains of A. draco and A.peninsulae and were analyzed by isoelectric focusing with PAGE. Distinct interspecific differences were found in EST and SOD electrophoregrams,but not found in LDH electrophoregrams.It was suggested that A.draco and A.peninsulae could be unequivocally distinguished by the presence or absence of A 2 band in EST isozymes or by the variations of isoelectric point(pI)of main bands in SOD isozymes.