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| 疏水层析纯化人重组白细胞介素－4 | |
| 引用本文： | 戴燕,李燕,刘洁,王宏,陈慰峰. 疏水层析纯化人重组白细胞介素－4[J]. 中国生物化学与分子生物学报, 1996, 12(1): 83-87 |
| 作者姓名： | 戴燕 李燕 刘洁 王宏 陈慰峰 |
| 作者单位： | 北京医科大学免疫室 |
| 摘 要： | 应用疏水层析对大肠杆菌表达的人重组白细胞介素－４（ｒｈＩＬ－４）进行了纯化，含有ｒｈＩＬ－４的包涵体，经洗涤、变性、复性后，以Ｂｕｔｙｌ－Ｓｅｐｈａｒｏｓｅ层析，得到了高纯度的ｒｈＩＬ－４．纯度达９７％；回收率为３２％；比活性为２×１０~７Ｕ／ｍｇ，讨论了ｒｈＩＬ－４疏水层析的条件，并对不同的方法纯化白细胞介素－４进行了比较． |
| 关 键 词： | 人重组白细胞介素－4 疏水层析 蛋白质纯化 |
| 收稿时间： | 1996-02-20 |
Purification of rhIL-4 by Hydrophobic Chromatography |
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| Dai Yan,Li Yan,Liu Jie,Wang Hong,Chen Wei-Feng. Purification of rhIL-4 by Hydrophobic Chromatography[J]. Chinese Journal of Biochemistry and Molecular Biology, 1996, 12(1): 83-87 | |
| Authors: | Dai Yan Li Yan Liu Jie Wang Hong Chen Wei-Feng |
| Affiliation: | (Department of Immunology, Beijing Medical Unicersity, Beijing 100083 |
| Abstract: | A protocol of hydrophobic chromotography for purifying rhIL-4 expressed in E. coli was reported. After washing, denaturing and refolding the inclusion bodies separated from E. coli, the purity of the rhIL-4 obtained from this step was 97 %. The overall recovery of rhIL-4 was 32% and its specific activity was 2 ×10￣7 U/ mg.The consequence of phenyl-sepharose chromatography was showed and the optimal conditions and efficiency for purifying rhIL-4 by hydrophobic chromatography were discussed. Also compared hydrophobic protocol with other purification protocols. |
| Keywords: | Recombinant human interleukin-4(rhIL-4) Hydrophobic chromatography Purification of protein |
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