Immunohistological localization and quantification of IAA in studies of root growth regulation |
| |
| Authors: | L B Vysotskaya S Yu Veselov D S Veselov V N Filippenko E A Ivanov I I Ivanov G R Kudoyarova |
| |
| Institution: | (1) Institute of Biology, Ufa Research Center, Russian Academy of Sciences, pr. Oktyabrya 69, Ufa, Bashkortostan, 450054, Russia;(2) Department of Biology, Bashkortostan State University, ul. Frunze 32, Ufa, 450074, Russia |
| |
| Abstract: | The content and distribution of auxins were studied in gravistimulated roots of maize (Zea mays L.) and primary roots of 7-day-old wheat (Triticum durum Desf.) seedlings, which branching was enhanced by excision of adventitious roots. IAA localization was observed immunohistochemically, using specific anti-IAA antibody in combination with second (anti-species) antibody labeled with colloidal gold. Differences in the IAA content (staining intensity) were found between upper and lower parts of gravistimulated maize roots. We also observed IAA accumulation in the primary wheat root after adventitious root excision; the cells of lateral root primordia were characterized by more intense IAA staining. The role of auxin redistribution in plants for lateral root initiation and development is discussed. |
| |
| Keywords: | Zea mays Triticum durum auxins lateral roots immunohistichemical localization |
| 本文献已被 SpringerLink 等数据库收录！ |
|
