Globular embryo-like structures and highly efficient thidiazuron-induced multiple shoot formation in saffron (<Emphasis Type="Italic">Crocus sativus</Emphasis> L.) |
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Authors: | Golandam Sharifi Hassan Ebrahimzadeh Behzad Ghareyazie Mansour Karimi |
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Institution: | (1) Laboratory of Plant Physiology, Department of Botany, Faculty of Science, University of Tehran, Tehran, Iran;(2) Department of Genomics, Agricultural Biotechnology Research Institute of Iran (ABRII), Seed and Plant Improvement Institutes Campus, P.O. Box 31535-1897, Mahdasht Road, Karaj, Iran;(3) Department of Plant Systems Biology, Flanders Institute for Biotechnology and Department of Molecular Genetics, Ghent University, 9052 Ghent, Belgium |
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Abstract: | Saffron (Crocus sativus L.) is a monocotyledonous plant propagated via corms, but recently several alternative methods have been reported. To find
the conditions suitable for saffron shoot formation from corms, the effect of different concentrations of the plant growth
regulatory cytokinins N6-benzyladenine (BA) and N-phenyl-1, 2,3-thidiazol-5-ylurea, commonly known as thidiazuron (TDZ), were compared. In all corm
explants, an average of 39.5 ± 5.1 shoots per corm were induced by 4.54 μM TDZ, whereas only 3.6-11.4% by BA. The outstanding
result in the shoot formation stage is the generation of globular, translucent structures that are morphologically similar
to globular embryos. To optimize the plant regeneration from the induced adventitious shoots obtained from the TDZ treatment,
the shoots were transferred to MS and B5 media supplemented with different concentrations and combinations of NAA and BA.
The highest rate of plant regeneration from developing shoots was observed in the B5 medium containing 2.22 μM NAA and 2.68 μM
BA. With optimized hormonal conditions, an average of 19.55 ± 5.75 shoots and 3.18 ± 1.5 roots per explants were obtained.
Based on this experiment, a simple, new and efficient protocol is presented to produce numerous plants from induced corm explants
of saffron. |
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