A pitfall in using BODIPY dyes to label lipid droplets for fluorescence microscopy |
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Authors: | Yuki Ohsaki Yuki Shinohara Michitaka Suzuki Toyoshi Fujimoto |
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Institution: | (1) Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa, Nagoya 466-8550, Japan; |
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Abstract: | The lipid droplet (LD) has become a focus of intense research. Fluorescence labeling is indispensable for the cell biological
analysis of the LD, and a lipophilic fluorescence dye, BODIPY 493/503, which emits bright green fluorescence has been used
extensively for LD labeling. The dye is convenient for double fluorescence labeling, but we noticed that it emits red fluorescence
under certain conditions, which could lead to erroneous interpretations. We propose a protocol to preclude such a possibility. |
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Keywords: | |
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