Transfer RNA gene mapping studies on chloroplast DNA from Chlamydomonas reinhardii |
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| Authors: | P Bergmann M Schneider G Burkard JH Weil JD Rochaix |
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| Institution: | 1. Institut de Biologie Moléculaire et Cellulaire, Université Louis Pasteur, 15 Rue Descartes, 67084 Strasbourg France;2. Département de Biologie Moléculaire, Université de Genève, 30 Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland |
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| Abstract: | Total tRNA of Chlamydomonas reinhardii was fractionated by 2-dimensional gel electrophoresis. Sixteen tRNAs specific for eleven amino acids could be identified by aminoacylation with Escherichia coli tRNA synthetases. Hybridization of these tRNAs with chloroplast restriction fragments allowed for the localization of the genes of tRNATyr, tRNAPro, tRNAPhe (2 genes), tRNAIle (2 genes) and tRNAHis (2 genes) on the chloroplast genome of C. reinhardii. The genes for tRNAAla (2 genes), tRNAAsn and tRNALeu were mapped by using individual chloroplast tRNAs from higher plants as probes. |
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| Keywords: | tRNA chloroplast DNA |
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