The development of the region between the preexisting and nascent membranes during the first cleavage of Rana amurensis eggs

The region between the preexisting and nascentmembranes of a cleaving Rana egg is a dense protrusionregion of nearly 40μm wide at 180°stage.Later,thisregion differentiate,into an upper part,a strip withlong, branched and randomly distributed protrusionswhich are derived largely from the preexistingmembrane,and a lower part,a band with microvilli.During 4-and 8-cel1 stages,the strip is almost vanishedand microvilli in the band is shortened.The nascentmembrane is smooth at the 180°stage,then a rough areaappears below the smooth region and quickly expands.Wheat germ agglutinin which can bind to preexistingmembrane interrupts the development of the regionbetween the preexisting and nascent membranes.Detergent,Brij,having the property to increase the areaof nascent membrane,does not interrupt the developmentof the region between the preexisting and nascentmembranes.

The development of the region between the preexisting and nascent membranes during the first cleavage of Rana amurensis eggs

The region between the preexisting and nascent membranes of a cleaving Rana egg is a dense protrusion region of nearly 40 um wide at 180 stage. Later, this region differentiate, into an upper part, a strip with long, branched and randomly distributed protrusions which are derived largely from the preexisting membrane, and a lower part, a band with miorovilli. During 4-and 8-cell stages, the strip is almost vanished and miorovilli in the band is shortened. The nascent membrane is smooth at the 180 stage, then a rough area appears below the smooth region and quickly expands. Wheat germ agglutinin which can bind to preexisting membrane interrupts the development of the region between the preexisting and nascent membranes. Detergent, Brij, having the property to increase the area of nascent membrane, does not interrupt the development of the region between the preexisting and nascent membranes.