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Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay |
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| Authors: | Philippe Le Flèche Isabelle Jacques Maggy Grayon Sascha Al Dahouk Patrick Bouchon France Denoeud Karsten Nöckler Heinrich Neubauer Laurence A Guilloteau Gilles Vergnaud |
| Institution: | (1) Centre d'Etudes du Bouchet BP3, 91710 Vert le Petit, France;(2) GPMS, Bt. 400, Institut de Gntique et Microbiologie, Universit Paris Sud, 91405 Orsay cedex, France;(3) UR918 Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France;(4) Institut Universitaire de Technologie, 29 rue du Pont Volant, 37082 Tours Cedex 2, France;(5) Dept. of Bacteriology, Bundeswehr Institute of Microbiology, Neuherbergstr. 11, D-80937, Munich, Germany;(6) Federal Institute for Risk Assessment, BfR, Diedersdorfer Weg 1, D-12277, Berlin, Germany |
| Abstract: | BackgroundThe classification of Brucella into species and biovars relies on phenotypic characteristics and sometimes raises difficulties in the interpretation of the results due to an absence of standardization of the typing reagents. In addition, the resolution of this biotyping is moderate and requires the manipulation of the living agent. More efficient DNA-based methods are needed, and this work explores the suitability of multiple locus variable number tandem repeats analysis (MLVA) for both typing and species identification. |
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