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The effects of Sp7/Osterix gene silencing in the chondroprogenitor cell line, ATDC5 |
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| Authors: | Omoteyama Kazuki Takagi Minoru |
| Affiliation: | a Department of Plant Science, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea b Plant Genomics and Breeding Institute, Seoul National University, Seoul 151-921, Republic of Korea c Bio-MAX Institute, Seoul National University, Seoul 151-818, Republic of Korea d Laboratory of Plant Molecular Biology, Rockefeller University, NY 10065, USA e Boyce Thompson Institute for Plant Research, Ithaca, NY 14853, USA f School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Republic of Korea |
| Abstract: | Ubiquitin is a small polypeptide and ubiquitination is the post-translational modification by ubiquitin protein, resulting in degradation of target proteins by the 26S proteasome complex. Here, we found that E3 ubiquitin ligase SINAT5, an Arabidopsis homologue of the Drosophila SINA RING-finger protein, interacts directly with LHY, a component of the circadian oscillator, and DET1, a negative regulator of light-regulated gene expression. We also found that SINAT5 has E3 ubiquitination activity for LHY but not for DET1. Interestingly, LHY ubiquitination by SINAT5 was inhibited by DET1. Late flowering of sinat5 mutants indicates that flowering time can be controlled by DET1 through regulation of LHY stability by SINAT5. |
| Keywords: | E1, ubiquitin-activating enzyme E2, ubiquitin-conjugating enzyme E3, ubiquitin ligase HECT, E6-associated protein carboxyl terminus SINA, seven in absentia SINAT5, an Arabidopsis homologue of the SINA Siah1, a human homologue of the SINA NAC1, NOAPICAL MERISTEM/CUP-SHAPED COTYLEDON 1 SINAT5DN, a dominant-negative Cys-49/Ser RING domain mutant of SINAT5 FLC, FLOWERING LOCUS C DET1, DE-ETIOLATED1 DDB1, ultraviolet-damaged DNA-binding protein 1 LHY, LATE ELONGATED HYPOCOTYL TOC1, TIMING OF CAB1 PCR, polymerase chain reaction GST, glutathione S-transferase IPTG, isopropyl-β- smallcaps" >d-thiogalactoside 3-AT, 3-amino-1,2,4-triazole SN, N-terminal fragment encoding the amino acids 1-158 of SINAT5 SC, C-terminal fragment encoding the amino acids 156-310 of SINAT5 SR, N-terminal fragments encoding the RING finger motif (amino acids 1-100) SZ, N-terminal fragments encoding the zinc finger motif (amino acids 83-182) |
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