In vitro development of bovine secondary follicles in two- and three-dimensional culture systems using vascular endothelial growth factor,insulin-like growth factor-1, and growth hormone |
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| Affiliation: | 1. Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, Illinois, USA;2. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Ceará, Brazil;1. Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, lllinois, USA;2. Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq;3. Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS, USA;1. Transgenesis Center of Excellence, Isfahan (Khorasgan) Branch, Islamic Azad University, Iran;2. Department of Animal Science, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran;1. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran;2. Department of Biomedical Engineering, University of Virginia, Charlottesville, VA 22908, USA;3. Department of Cell Biology, Center for Research in Contraceptive and Reproductive Health, University of Virginia, Charlottesville, VA 22908, USA;4. Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran;5. Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran;6. Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran;1. Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA;2. Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA;3. Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA;4. Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA;1. Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil;2. Institute of Health Sciences, University of International Integration Lusophone African-Brazilian, Acarape, CE, Brazil;3. Federal University of Uberlândia, MG, Brazil;4. Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, OR, USA |
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| Abstract: | The aim of this study was to evaluate the development and estradiol production of isolated bovine secondary follicles in two-dimensional (2D, experiment 1) and three-dimensional (3D using alginate, experiment 2) long-term culture systems in the absence (control group; only α-MEM+) or presence of vascular endothelial growth factor (VEGF), insulin-like growth factor-1, or GH alone, or a combination of all. A total of 363 isolated secondary follicles were cultured individually for 32 days at 38.5 °C in 5% CO2 in a humidified incubator with addition of medium (5 μL) every other day. In 2D culture system, follicular growth and antrum formation rates were higher (P < 0.05) in VEGF treatment compared with the other treatments. In 3D culture system, only estradiol concentration was greater (P < 0.05) in the GH than in the control group, whereas the other end points were similar (P > 0.05). In summary, this study demonstrated that the benefits of using a certain type of medium supplement depended on the culture system (2D vs. 3D). Vascular endothelial growth factor was an effective supplement for the in vitro culture of bovine secondary follicles when the 2D culture system was used, whereas GH only affected estradiol production using the 3D culture system. This study sheds light on advancements in methodology to facilitate subsequent studies on bovine preantral follicle development. |
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| Keywords: | Cattle Culture systems Estradiol Growth factors Preantral follicles |
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