Sago starch as a low-cost carbon source for exopolysaccharide production by Lactobacillus kefiranofaciens |
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Authors: | Chittra Yeesang Supitchaya Chanthachum Benjamas Cheirsilp |
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Institution: | (1) Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand;(2) Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand |
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Abstract: | Low-cost sago starch was used as a carbon source for production of the exopolysaccharide kefiran by Lactobacillus kefiranofaciens. A simultaneous saccharification and fermentation process of sago starch for kefiran production was evaluated. Factors affecting
the process such as an initial pH, temperature, starch concentration, including a mixture of α-amylase and glucoamylase were
determined. The highest kefiran concentration of 0.85 g/l was obtained at the initial pH of 5.5, temperature of 30 °C, starch
concentration of 4% and mixed-enzymes with activity of 100 U/g-starch. The use of a mixture of α-amylase and glucoamylase
could enhance the productivity compared to the use of α-amylase alone. The optimal ratio of α-amylase to glucoamylase of 60:40
gave the highest kefiran production rate of 11.83 mg/l/h. This study showed that sago starch could serve as a low-cost substrate
for kefiran production. |
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Keywords: | Exopolysaccharide Kefiran Sago starch Simultaneous saccharification and fermentation |
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