|
|||||
|
|
Genetic variation and relationships in Laetiporus sulphureus s. lat., as determined by ITS rDNA sequences and in vitro growth rate |
|
| Authors: | Rimvydas Vasaitis Audrius Menkis Soonja Seok Libor Jankovsky Bernard Slippers |
| Institution: | a Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, P.O. Box 7026, SE-750 07 Uppsala, Sweden b National Institute of Biological Resources, Gyungseo-dong, Seo-gu, Incheon, 404-708, Republic of Korea c National Institute of Agricultural Sciences and Technology, Rural Development Administration (RDA), Suwon-si, 441-707, Republic of Korea d Department of Forest Protection and Wildlife Management, Mendel University of Agriculture and Forestry in Brno, Zemedelska 3, CZ-61300 Brno, Czech Republic e Laboratory of Phytopathogenic Microorganisms, Institute of Botany, Zaliuju Ezeru str. 49, LT-08406 Vilnius, Lithuania f Department of Genetics, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria 0002, South Africa |
| Abstract: | The aim of this study was to characterise the genetic variation and molecular relationships of the brown rot polypore, Laetiporus sulphureus s. lat., from Europe, South America, Africa, and Asia, using ITS sequences of the nu-rDNA and by comparing the growth rate in vitro. In a NJ analysis of the sequences of 130 individuals of L. sulphureus s. lat., eight distinct clusters emerged, supported by BS values of 70-100 %. Within each cluster, the ITS rDNA sequence variation was below 3 %. The sequences were also analysed together with Laetiporus sequences available from GenBank. Results demonstrated the possible presence of L. huroniensis in Europe (invalidly named L. montanus) and L. gilbertsonii in South America, and provided more information on the Pan-American and European distribution of one of the clades, currently known in North America as L. sulphureus. L. conifericola formed a separate distinct clade. Moreover, the analysis revealed two unknown Laetiporus taxa in Korea, one in South Africa, and one in Europe. As L. sulphureus is described from Europe (France), and we show that more than one taxon exist here, it is presently not possible to define L. sulphureus s. str. Certain biological differences between some of the clades (in vitro growth rates, chemical composition, and pigmentation) were demonstrated and discussed. |
| Keywords: | Aphyllophorales Basidiomycota Laetiporus montanus Polyporaceae Tree decay Wood decomposition |
| 本文献已被 ScienceDirect 等数据库收录！ | |