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Radioactive labeling techniques for the identification of G antigen in the human Rh blood group system |
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| Authors: | Jacky Lirochon Charles Salmon Philippe Rouger Christian Doinel |
| Institution: | INTS (Fondation Nationale de Transfusion Sanguine), Paris, France. |
| Abstract: | The G antigen is one of the erythrocyte membrane Rh antigens. The amount of Rh antigen present on the red blood cell is about 10(-15) g and radioactive labeling of membrane proteins is a useful method for its identification and characterization. In this paper, we compare 4 labeling techniques. Using a human monoclonal anti-Rh(G) antibody and an immunofixation technique, we located the G antigen on a polypeptide of an average molecular weight of 28,000 Da. |
| Keywords: | erythrocyte labeling monoclonal anti-Rh(G) antibody Rh(G) antigen Rh blood groupAuthor Keywords: marquage é rythrocytaire anticorps monoclonal anti-G biochimie de l'antigè ne G systè me de groupe sanguin humain Rh |
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