| 牦牛病毒性腹泻病毒E2基因的克隆鉴定及原核表达 |
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| 引用本文： | 孙凯,刘亚刚,王妍,王盼盼,胡炳峰,王文伯. 牦牛病毒性腹泻病毒E2基因的克隆鉴定及原核表达[J]. 四川动物, 2011, 30(6): 886-889. DOI: 10.3969/j.issn.1000-7083.2011.06.008 |
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| 作者姓名： | 孙凯 刘亚刚 王妍 王盼盼 胡炳峰 王文伯 |
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| 作者单位： | 西南民族大学生命科学与技术学院,成都,610041 |
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| 基金项目： | 四川省科技厅攻关项目(04JY029-006-04); 国家民委项目(07XN03) |
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| 摘 要： | 运用聚合酶链式反应,以牦牛BVDV基因组DNA为模板扩增出牦牛BVDV E2基因.为研究E2蛋白的抗原性,将E2基因插入到pET-32a原核表达载体,构建重组表达质粒pET-32a-E2,并转化至BL21(DE3)宿主菌中,利用IPTG诱导表达.经SDS-PAGE检测,pET-32a-E2在宿主菌BL21(DE3)中表...
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| 关 键 词： | 牦牛 E2基因 牛病毒性腹泻病毒 原核表达 |
Cloning and Prokaryotic Expression of E2 Genes of Bovine Viral DiarrheaVirus Strain from Yak |
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| SUN Kai,LIU Ya-gang,WANG Yan,WANG Pan-pan,HU Bing-feng,WANG Wen-bo. Cloning and Prokaryotic Expression of E2 Genes of Bovine Viral DiarrheaVirus Strain from Yak[J]. Sichuan Journal of Zoology, 2011, 30(6): 886-889. DOI: 10.3969/j.issn.1000-7083.2011.06.008 |
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| Authors: | SUN Kai LIU Ya-gang WANG Yan WANG Pan-pan HU Bing-feng WANG Wen-bo |
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| Affiliation: | SUN Kai,LIU Ya-gang*,WANG Yan,WANG Pan-pan,HU Bing-feng,WANG Wen-bo(School of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,China) |
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| Abstract: | E2 gene was successfully amplified by PCR using genome DNA of the wild yak as template.In order to study the antigenicity of E2 protein,we inserted E2 gene into pET-32a vector and reconstructed plasmid pET-32a-E2 and then transformed them into BL21(DE3) host bacterium,using IPTG to induce them to express in the end.The result showed that a 58 kD fusion protein which was detected by SDS-PAGE was expressed efficiently by pET-32a-E2 in the BL21(DE3) host bacterium and the size of expressed protein was consiste... |
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| Keywords: | yak E2 gene bovine viral diarrhea virus prokaryotic expression |
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