Effects of methyl jasmonate and salicylic acid on cell growth and cryptotanshinone formation in Ti transformed Salvia miltiorrhiza cell suspension cultures |
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Authors: | Hui Chen Feng Chen |
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Institution: | (1) Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong |
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Abstract: | Exogenous methyl jasmonate (MJ) or salicylic acid (SA) when applied alone failed to induce cryptotanshinone (a phytoalexin) formation in Ti-transformed Salvia miltiorrhiza cell suspension cultures. However, when applied in combination with yeast elicitor, SA at 50–500 M enhanced the yeast elicitor-induced cryptotanshinone formation while MJ reduced the yeast elicitor-induced cryptotanshinone formation. Ibuprofen at 100 M did not inhibit the yeast elicitor-induced cryptotanshinone formation. DMSO was superior to ethanol as a solvent for introducing MJ to the cell cultures. |
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Keywords: | cryptotanshinone ibuprofen methyl jasmonate salicylic acid Salvia miltiorrhiza |
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