Modulatory efficacy of dieckol on xenobiotic-metabolizing enzymes,cell proliferation,apoptosis, invasion and angiogenesis during NDEA-induced rat hepatocarcinogenesis |
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Authors: | Velayutham Sadeeshkumar Arul Duraikannu Samuthrapandian Ravichandran Paulrasu Kodisundaram Wilson Sylvester Fredrick Rajagopal Gobalakrishnan |
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Institution: | 1.Department of Hepatopathy,Liaocheng People’s Hospital,Liaocheng,China |
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Abstract: | Altered microRNA expression is associated with tumor proliferation, metastasis, and tumorigenesis. In this study, we studied the role of miR-3117 in hepatocellular carcinoma (HCC) cell proliferation and found that miR-3117 was upregulated in HCC tissues and cells. MTT assay, soft agar growth assay, BrdU assay, and cell cycle assay revealed that miR-3117 overexpression promoted HCC HepG2 cell proliferation and that knockdown of miR-3117 suppressed HepG2 proliferation. Mechanism analysis suggested PH domain and leucine-rich repeat protein phosphatase-like (PHLPPL) as the target of miR-3117. Luciferase reporter assay suggested that miR-3117 directly binds to the 3′UTR of PHLPPL. Double knockdown of miR-3117 and PHLPPL copied the phenotypes caused by miR-3117 overexpression, suggesting that miR-3117 contributes to the proliferation of HepG2 by targeting PHLPPL. Our study provided a target for HCC therapy. |
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