A halotolerant and thermotolerant <Emphasis Type="Italic">Bacillus</Emphasis> sp. degrades hydrocarbons and produces tensio-active emulsifying agent |
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Authors: | Manoj Kumar Vladimir León Angela De Sisto Materano Olaf A Ilzins |
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Institution: | (1) Petroleum Biotechnology Unit, Center of Biotechnology, Foundation Institute of Advance Studies (IDEA), Caracas, Venezuela;(2) Present address: Indian Oil Corporation, Research and Development Center, Faridabad, 121007, Haryana, India |
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Abstract: | A Bacillus sp. strain DHT, isolated from oil-contaminated soil, grew and produced biosurfactant when cultured in variety of substrate
at salinities of up to 100 g l−1 and temperatures up to 45°C. It was capable of utilizing crude oil, fuels, various pure alkanes and PAHs as a sole carbon
and energy source across a wide range of temperature and salinity. Over the range evaluated, the degradation of hydrocarbon
and biosurfactant production was not influenced by salinity (0–10% wv−1) and temperature (30–45°C). The biosurfactant produced by the organism emulsified a range of hydrocarbons with hexadecane
as the best substrate and toluene as the poorest. From 16S rDNA analysis, strain DHT was related to Bacillus licheniformis. |
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Keywords: | Bacillus licheniformis Biosurfactant Halotolernat Themotolerant Hydrocarbon biodegradation |
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