| RAPD分析在绢丝昆虫亲缘关系研究中的应用Ⅱ.柞蚕品种间的遗传差异 |
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| 引用本文： | 桂慕燕,左正宏,王学民,陈元霖GUI Mu-yan,ZUO Zheng-hong,WANG Xue-min,CHEN Yuan-lin.RAPD分析在绢丝昆虫亲缘关系研究中的应用Ⅱ.柞蚕品种间的遗传差异[J].遗传,2001,23(5):452-646. |
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| 作者姓名： | 桂慕燕 左正宏 王学民 陈元霖GUI Mu-yan ZUO Zheng-hong WANG Xue-min CHEN Yuan-lin |
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| 作者单位： | 厦门大学生命科学学院福建厦门 361005
Dept.of Bio.Xiamen University,Xiamen,FujianProvince,361005 China |
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| 摘 要： | 采用RAPD技术,对5个柞蚕品种的遗传差异进行比较研究.结果表明,所采用的40个随机引物中,有27个引物扩增谱带清晰且重复性较好,扩增总片段数253条,单个引物的扩增片段数在4～16之间,片段大小在0.33～3.0kb之间.不同柞蚕品种间的遗传差异较小,遗传距离(D)在0.066～0.1659之间,根据D值,由UPGMA聚类分析软件绘制了它们的分子进化树。
Abstract:Random amplified Polymorphic DNA (RAPD) was used to analyze the genetic diversity among Antheraea pernyi.The genetic variance of five Antheraea pernyi was studied.The result showed that:27 of 40 arbitrary primers could amplify clear and repeating bands.A total of 262 fragments were obtained.Each primer gave 4~16 bands and the average was 9.7.The length of the band was 0.33~3.0kb.The D value between different breeds of Antheraea pernyi was 0.066~0.1659.The D value was used to construct a dendrogram by UPGMA.
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| 关 键 词： | 柞蚕 RAPD Antheraea pernyi 品种差异 Key words RAPD |
Application of RAPD Technique in Genetic Relationship of Silk Insect II. Genetic Variance in Antheraea pernyi |
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| GUI Mu-yan,ZUO Zheng-hong,WANG Xue-min,CHEN Yuan-lin.Application of RAPD Technique in Genetic Relationship of Silk Insect II. Genetic Variance in Antheraea pernyi[J].Hereditas,2001,23(5):452-646. |
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| Authors: | GUI Mu-yan ZUO Zheng-hong WANG Xue-min CHEN Yuan-lin |
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