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A 3-Mb Sequence-Ready Contig Map Encompassing the Multiple Disease Gene Cluster on Chromosome 11q13.1-q13.3 |
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| Authors: | Kitamura Eiko; Hosoda Fumie; Fukushima Michiyo; Asakawa Shuichi; Shimizu Nobuyoshi; Imai Takashi; Soeda Eiichi; Ohki Misao |
| Institution: | 1Radiobiology Division, National Cancer Center Research Institute 5-1-1 Tsukiji, Chuo-ku, Tokyo 104, JAPAN 2Department of Molecular Biology, Keio University School of Medicine Shinjuku-ku, Tokyo 160, JAPAN 3Genome Research Group, National Institute of Radiological Science 4-9-1 Anagawa, Inage-ku, Chiba 263, JAPAN 4RIKEN Gene Bank, The Institute of Physical and Chemical Research 3-1-1 Koyadai, Tsukuba, 305, JAPAN |
| Abstract: | Despite the presence of several human disease genes on chromosome11q13, few of them have been molecularly cloned. Here, we reportthe construction of a contig map encompassing 11q13.1–q13.3using bacteriophage P1 (P1), bacterial artificial chromosome(BAC), and P1-derived artificial chromosome (PAC). The contigmap comprises 32 P1 clones, 27 BAC clones, 6 PAC clones, and1 YAC clone and spans a 3-Mb region from D11S480 to D11S913.The map encompasses all the candidate loci of Bardet-Biedlesyndrome type I (BBS1) and spinocerebellar ataxia type 5 (SCA5),one-third of the distal region for hereditary paraganglioma2 (PGL2), and one-third of the central region for insulin-dependentdiabetes mellitus 4 (IDDM4). In the process of map construction,61 new sequence-tagged site (STS) markers were developed fromthe Not I linking clones and the termini of clone inserts. Wehave also mapped 30 ESTs on this map. This contig map will facilitatethe isolation of polymorphic markers for a more re.ned analysisof the disease gene region and identi.cation of candidate genesby direct cDNA selection, as well as prediction of gene functionfrom sequence information of these bacterial clones. |
| Keywords: | chromosome 11 contig SCA5 BBS1 |
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