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A physical map of the Chinese chestnut (Castanea mollissima) genome and its integration with the genetic map |
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| Authors: | Guang-Chen Fang Barbara P. Blackmon Margaret E. Staton C. Dana Nelson Thomas L. Kubisiak Bode A. Olukolu David Henry Tatyana Zhebentyayeva Christopher A. Saski Chun-Huai Cheng Megan Monsanto Stephen Ficklin Michael Atkins Laura L. Georgi Abdelali Barakat Nicholas Wheeler John E. Carlson Ronald Sederoff Albert G. Abbott |
| Affiliation: | 1. USDA APHIS BRS, 920 Main Campus Drive, Suite 200, Raleigh, NC, 27606, USA 2. Clemson University Genomics Institute, Clemson University, BRC, 105 Collings Street, Clemson, SC, 29634, USA 3. Southern Institute of Forest Genetics, U.S. Forest Service, 23332 Success Road, Saucier, MS, 39574, USA 4. Department of Plant Pathology, North Carolina State University, Raleigh, NC, 27695, USA 5. Piedmont Technical College, 620 North Emerald Road, Greenwood, SC, 29648, USA 6. Department of Genetics and Biochemistry, Clemson University, 100 Jordan Hall, Clemson, SC, 29634, USA 7. Department of Horticulture, Washington State University, Johnson Hall 149, Pullman, WA, 99164, USA 8. SDSU Heart Institute and Department of Biology, San Diego State University, 5500 Campanile Drive, San Diego, CA, 92182, USA 9. Meadowview Research Farms, The American Chestnut Foundation, 29010 Hawthorne Drive, Meadowview, VA, 24361, USA 11. The School of Forest Resources, The Huck Institutes of the Life Sciences, Pennsylvania State University, 324 Forest Resources Building, University Park, PA, 16802, USA 10. Department of Forestry and Environmental Resources, North Carolina State University, Campus Box 8001, Raleigh, NC, 27695, USA 12. Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Campus Box 7247, Raleigh, NC, 27695, USA |
| Abstract: | Three Chinese chestnut bacterial artificial chromosome (BAC) libraries were developed and used for physical map construction. Specifically, high information content fingerprinting was used to assemble 126,445 BAC clones into 1,377 contigs and 12,919 singletons. Integration of the dense Chinese chestnut genetic map with the physical map was achieved via high-throughput hybridization using overgo probes derived from sequence-based genetic markers. A total of 1,026 probes were anchored to the physical map including 831 probes corresponding to 878 expressed sequence tag-based markers. Within the physical map, three BAC contigs were anchored to the three major fungal blight-resistant quantitative trait loci on chestnut linkage groups B, F, and G. A subset of probes corresponding to orthologous genes in poplar showed only a limited amount of conserved gene order between the poplar and chestnut genomes. The integrated genetic and physical map of Chinese chestnut is available at www.fagaceae.org/physical_maps. |
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