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In vitro modification of the chemical composition of human plasma low density lipoproteins: effects of morphology and thermal properties |
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| Authors: | R Zechner G M Kostner H Dieplinger G Degovics P Laggner |
| Affiliation: | 1. Institute of Medical Biochemistry, University of Graz, Steyrergasse 17, A-8010 Graz Austria;2. Institut für Roentgenfeinstrukturforschung der Oesterreichischen Akademie der Wissenschaften und des Forschungszentrums Graz, Steyrergasse 17, A-8010 Graz Austria |
| Abstract: | The effects of enzymatic action on human low density lipoproteins (LDL) occurring during in vitro incubation of plasma have been studied by chemical analysis, analytical ultracentrifugation, negative stain electron microscopy and X-ray small angle scattering. Chemically, the action of cholesteryl ester exchange and transfer proteins(s) (CEPT) leads to a relative increase in trigylcerides at the expense of cholesteryl esters. Morphologically, the particles maintain their characteristic features detectable by X-ray small angel scattering. Additional action of lecithin/cholesterol acyl transferase (LCAT) causes mainly a decrease in polar lipid contents and a reduction in particle size. The associated changes in the thermotropic transition were found to be strongly correlated to the triglyceride/cholesteryl ester ratio. |
| Keywords: | CETP cholesteryl ester transfer protein HDL high density lipoproteins LCAT lecithin-cholesterol acyl transferase LDL low density lipoproteins VLDL very low density lipoproteins low density lipoproteins lecithin/cholesterol acyl transferase cholesteryl ester transfer protein X-ray scattering thermal transition |
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