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Mucor hiemalis endo-beta-N-acetylglucosaminidase can transglycosylate a bisecting hybrid-type oligosaccharide from an ovalbumin glycopeptide |
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| Authors: | Osumi Kenji Makino Yoshitaka Akaike Eri Yamanoi Takashi Mizuno Mamoru Noguchi Midori Inazu Toshiyuki Yamamoto Kenji Fujita Kiyotaka |
| Institution: | The Noguchi Institute, 1-8-1 Kaga, Itabashi-ku, Tokyo 173-0003, Japan. |
| Abstract: | We found that the recombinant endo-beta-N-acetylglucosaminidase of Mucor hiemalis (Endo-M) expressed in Candida boidinii had the transglycosylation activity of transferring a bisecting hybrid-type oligosaccharide from an ovalbumin glycopeptide to the acceptor (p-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside) in a good yield of 43%. |
| Keywords: | endo-β-N-Acetylglucosaminidase Endo-M Transglycosylation Bisecting hybrid-type oligosaccharide |
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