Mouse nerve growth factor: a rapid isolation procedure for the alpha and gamma subunits. |
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| Authors: | I Jeng R Y Andres R A Bradshaw |
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| Institution: | Department of Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110 USA |
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| Abstract: | A rapid method for isolating the α and γ subunits of mouse submaxillary gland nerve growth factor, as by-products of the commonly used Bocchini-Angeletti (2.5 S) procedure, has been devised. Approximately 40 mg of each subunit is obtained from 400 pairs of adult glands. The subunits isolated in this fashion are indistinguishable from those obtained from the homogeneous 7 S complex as judged by gel electrophoresis or their association-dissociation behavior. |
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