硝基左旋精氨酸对睡眠抑制作用的机制研究

本文观察了硝基左旋精氨酸（Ｌ－ＮＮＡ，５０ｍｇ／ｋｇ，ｉｐ）和Ｌ－精氨酸（Ｌ－ａｒｇ，１１０ｍｇ／ｋｇ，ｉｐ）对慢性植入电极的大鼠睡眠－觉醒周期的影响及中缝核５－羟色胺（５－ＨＴ）神经元免疫阳性反应的变化。结果表明：Ｌ－ＮＮＡ显著抑制慢波睡眠和快眼动睡眠，使平均动脉压（ＭＡＰ）升高。Ｌ－ａｒｇ则使ＭＡＰ显著降低，对睡眠无明显影响。预先给予Ｌ－ａｒｇ可逆转Ｌ－ＮＮＡ的效应。腹腔给予Ｌ－ＮＮＡ后２ｈ，

A STUDY OF THE MECHANISM OF L-NNA ON SLEEP INHIBITI0N

In the present study, the sleep-waking cycle and the immunoreactivity of 5-HTcontainingneurons in rats receiving intraperitoneal injections of N-Nitro-L-arginine (L-NNA, 50 mg/kg) and L-arginine (L-arg, 110 mg/kg) were observed respectively.The results showed that injection of L-NNA suppressed both slow wave sleep (SWS) and rapid eye movement sleep (REMS) and increased mean arterial pressure (MAP). L-arg markedly attenuated MAP, whereas SWS and REMS were unchanged. The effects of L-NNA on sleep and MAP were reversed by preinjection of L-arg. In comparison with control group, the positive cells in dorsal raphe nucleus (DR) and raphe magUs nucleus (MnR ) increased 2 h a.fer injection of L-NNA. The effect was attenuated by preadministration of L-arg. All these results suggest that L-arg: NO pathway is involved in theregUlation of sleep-waking cycle and the increment of the positive immunoreactivity of the 5-HT-c0ntaining neurons in the DR may be related to the sleep-suppressive effects induced by L-NNA.