| 东海洋山港沿岸土壤、海水样本中Ⅰ型PKS基因的初步筛选鉴定与功能分析 |
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| 引用本文： | 董晓毅,王梁华,孙铭娟,宗 英,焦豫良,焦炳华.东海洋山港沿岸土壤、海水样本中Ⅰ型PKS基因的初步筛选鉴定与功能分析[J].微生物学通报,2008,35(9):1359-1366. |
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| 作者姓名： | 董晓毅 王梁华 孙铭娟 宗 英 焦豫良 焦炳华 |
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| 作者单位： | 中国人民解放军第二军医大学基础医学部生物化学与分子生物学教研室,上海,200433 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，国家自然科学基金 |
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| 摘 要： | 为考察土壤和海水中Ⅰ型聚酮合酶(polyketide synthase,PKS)基因的多样性和差异,本研究自行设计了一套针对PKS基因中酮缩合酶(ketosynthase,KS)片段的简并引物,使用PCR方法直接克隆东海洋山港沿岸土壤和海水DNA样本中的KS片段,去除重复序列后,共获得了23条不同的KS片段(长度为630 bp～690 bp),提交GenBank皆获登录号,其中19条来自土壤(DQ640993,DQ640997、DQ641926、DQ641927、DQ673137～DQ673151),4条来自海水(DQ673151,EF554859～EF554861),由核苷酸序列推断出的氨基酸序列保守,与GenBank中已知KS基因片段的相似度在45%到85%之间,种系发生分析表明,其中14条KS片段(来源于海水的KS片段皆在其中)应来源于典型的KS群,而剩余9条则来源于杂合的PKSmRPS(Non-ribosomalpeptide synthetase.非核糖体多肽合成酶)群.另外,几条KS基因特征明显,可用于进一步的研究.
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| 关 键 词： | 未获得培养微生物 聚酮合酶 酮缩合酶 土壤 海水 |
Screening, Identifying and Function Analysis of Polyketide Synthase I Domains from Soil and Seawater of Yangshan Harbor |
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| DONG Xiao-Yi,WANG Liang-Hu,SUN Ming-Juan,ZONG Ying,JIAO Yu-Liang and JIAO Bing-Hua.Screening, Identifying and Function Analysis of Polyketide Synthase I Domains from Soil and Seawater of Yangshan Harbor[J].Microbiology,2008,35(9):1359-1366. |
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| Authors: | DONG Xiao-Yi WANG Liang-Hu SUN Ming-Juan ZONG Ying JIAO Yu-Liang and JIAO Bing-Hua |
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| Institution: | Department of Biochemistry and Molecular Biology, Faculty of Basic Medical Science, Second Military Medical University, Shanghai 200433;Department of Biochemistry and Molecular Biology, Faculty of Basic Medical Science, Second Military Medical University, Shanghai 200433;Department of Biochemistry and Molecular Biology, Faculty of Basic Medical Science, Second Military Medical University, Shanghai 200433;Department of Biochemistry and Molecular Biology, Faculty of Basic Medical Science, Second Military Medical University, Shanghai 200433;Department of Biochemistry and Molecular Biology, Faculty of Basic Medical Science, Second Military Medical University, Shanghai 200433;Department of Biochemistry and Molecular Biology, Faculty of Basic Medical Science, Second Military Medical University, Shanghai 200433 |
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| Abstract: | |
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| Keywords: | Uncultured microbes Polyketide synthase Ketosyntase Soil Sea water |
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