Ergothioneine production with Aspergillus oryzae |
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| Authors: | Shun Takusagawa Iwao Ohtsu Tohru Dairi |
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| Institution: | 1. Graduate School of Chemical Science and Engineering, Hokkaido University, Sapporo, Hokkaido, Japan;2. Innovation Medical Research Institute, University of Tsukuba, Tsukuba, Ibaraki, Japan;3. Graduate School of Engineering, Hokkaido University, Sapporo, Hokkaido, Japan |
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| Abstract: | To establish a reliable and practical ergothioneine (ERG) supply, we employed fermentative ERG production using Aspergillus oryzae, a fungus used for food production. We heterologously overexpressed the egt-1 and -2 genes of Neurospora crassa in A. oryzae and succeeded in producing ERG (231.0 mg/kg of media, which was 20 times higher than the wild type). Abbreviations: ERG: ergothioneine; HER: hercynine; Cys-HER: hercynylcysteine-sulfoxide; SAM: S-adenosylmethionine; SAH: S-adenosylhomocysteine; l-His: l-histidine; l-Cys: l-cysteine; LC-ESI-MS: liquid chromatography-electrospray ionization-mass spectrometry |
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| Keywords: | Ergothioneine molecular breeding heterologous expression Aspergillus oryzae Neurospora crassa |
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