Temperature-induced structural changes of apo-lactoferrin and their functional implications: a molecular dynamics simulation study |
| |
Authors: | Carol Nhan Colin J Rix |
| |
Institution: | 1. School of Science, RMIT University, Bundoora Campus, Melbourne, Australia;2. School of Science, RMIT University, City Campus, Melbourne, Australia |
| |
Abstract: | Lactoferrin (Lf) is an iron-binding glycoprotein present in secretory fluids from human and bovine sources. Sequence alignment was employed to identify a region on the C-lobe of Lf capable of binding to bacterial cell surfaces, followed by all-atom explicit solvent molecular dynamics simulations to study the conformational changes of Lf after exposure to three processing temperatures: pasteurisation (72°C), spray drying (90°C) and ultra-high temperature (UHT) (127°C). Below 90°C, the simulations indicate relatively minor changes in overall protein structure. At UHT conditions (127°C), however, marked disruptions to protein structure were found as demonstrated by a substantial decrease in protein dimensions due to collapse in the inter-lobe region. There was also a marked increase in residue fluctuations in several regions of known functional importance, including antibacterial, iron-binding, and putative membrane binding regions, the latter of which is stabilised by a triplet of hydrophobic residues comprised of Leu446, Trp448 and Leu451 at low temperature, but which are disrupted under UHT conditions. A unique network analysis confirmed these results as demonstrated by large clusters of residues with increased dynamical correlation in the N-terminal lobe. |
| |
Keywords: | Lactoferrin heat treatment milk proteins molecular dynamics probiotics |
|
|