Genome-wide identification and characterization of novel microRNAs in seed development of soybean |
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| Authors: | Lan Yu Rongkai Guo Yeqin Jiang Xinghuo Ye Zhihong Yang |
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| Affiliation: | 1. College of Life Sciences, Huzhou University, Huzhou P.R. China;2. Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai China |
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| Abstract: | MicroRNAs (miRNAs) are important and ubiquitous regulators of gene expression in eukaryotes. However, the information about miRNAs population and their regulatory functions involving in soybean seed development remains incomplete. Base on the Dicer-like1-mediated cleavage signals during miRNA processing could be employed for novel miRNA discovery, a genome-wide search for miRNA candidates involved in seed development was carried out. As a result, 17 novel miRNAs, 14 isoforms of miRNA (isomiRs) and 31 previously validated miRNAs were discovered. These novel miRNAs and isomiRs represented tissue-specific expression and the isomiRs showed significantly higher abundance than that of their miRNA counterparts in different tissues. After target prediction and degradome sequencing data-based validation, 13 novel miRNA–target pairs were further identified. Besides, five targets of 22-nt iso-gma-miR393h were found to be triggered to produce secondary trans-acting siRNA (ta-siRNAs). Summarily, our results could expand the repertoire of miRNAs with potentially important functions in soybean. |
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| Keywords: | miRNA degradome sequencing ta-siRNA Glycine max |
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