Transformation of kiwifruit using the <Emphasis Type="Italic">ipt</Emphasis> gene alters tree architecture |
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Authors: | Chikako Honda Shinnosuke Kusaba Takaaki Nishijima Takaya Moriguchi |
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Institution: | (1) National Institute of Fruit Tree Science, Morioka Iwate, 020-0123, Japan;(2) National Institute of Fruit Tree Science, Tsukuba Ibaraki, 305-8605, Japan;(3) National Institute of Floricultural Science, Tsukuba Ibaraki, 305-8519, Japan |
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Abstract: | To manipulate the architecture of woody plants by controlling endogenous cytokinin levels, the isopentenyl transferase gene (ipt) from Agrobacterim
tumefaciens was introduced to kiwifruit using stable transformation. Consequently, eight transgenic lines were obtained. Transgenic shoots
harboring the ipt gene were recalcitrant to rooting under tissue-culture conditions; thus, their in vitro-cultivated shoots were directly grafted
onto potted wild-type kiwifruit seedlings to evaluate their morphological features, and three lines (tmr2-4, tmr2-G, tmr3-C)
were successfully grafted. The grafted transgenic plants had dwarfing and branching phenotypes, both of which are typical
features of cytokinin overproduction. In addition, the number of buds increased and internode length was shorter in the grafted
transgenic plants. The content of a precursor, trans-zeatin riboside, and an active cytokinin, trans-zeatin, increased in one transgenic line, in which the level of ipt gene expression was high, indicating that morphological changes were related to expression levels of the ipt gene and cytokinin content. Possibilities for potential utilization of the ipt gene in manipulating tree shape are discussed. |
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