Quantitation of six androgens by combined high performance liquid chromatography and radioimmunoassay |
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Authors: | Susan J Cook NC Rawlings RI Kennedy |
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Institution: | Department of Veterinary Physiological Sciences Western College of Veterinary Medicine University of Saskatchewan Saskatoon, Saskatchewan S7N OWO Canada |
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Abstract: | A technique was developed to separate six androgens (testosterone, dihydrotestosterone, androstenedione, 5α-androstane-3 α, 17β-diol, 5α-androstane-3β, 17β-diol, and androsterone) by high performance liquid chromatography prior to quantitation by specific radioimmunoassay systems. Methanol:water (60:40 v:v) was used as the solvent system with a C18 reversed-phase column. The method was verified and used to quantitate the androgens in serum from adult rams bled every 20 minutes for 6 hours and yearling bulls bled every 30 minutes for 8 hours. Concentrations of all 6 androgens varied in an episodic manner with testosterone being the dominant androgen. |
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