Purification of phycoerythrin from <Emphasis Type="Italic">Porphyra yezoensis</Emphasis> Ueda (Bangiales,Rhodophyta) using expanded bed absorption |
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Authors: | Jian-Feng Niu Zhang-Fan Chen Guang-Ce Wang Bai-Cheng Zhou |
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Institution: | (1) Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China;(2) College of Marine Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China; |
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Abstract: | R-phycoerythrin was purified by means of phenyl-sepharose expanded bed absorption and DEAE-sepharose ion-exchange chromatography
from Porphyra yezoensis, one of the largest and important aquaculture species in China. Final R-phycoerythrin preparation was characterized by purity
ratio above 4 and the homogeneity in native PAGE, respectively. The results of absorption spectrum, fluorescence spectrum
and SDS-PAGE were in agreement with previous reports on R-PE. The yield of R-phycoerythrin was 0.82 mg g−1 wet leafy gametophyte of P. yezoensis. This method is a high-protein recovery technology while reducing processing time, and is suitable for the large batch production
of R-phycoerythrin, which will enhance the value of P. yezoensis in China, especially the inferior P. yezoensis which can not be used for flake processing. |
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