Plant regeneration from protoplasts of wild rice (Oryza rufipogon Griff.) |
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| Authors: | Abdul Baset Robert P. Finch Edward C. Cocking |
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| Affiliation: | (1) Present address: Plant Breeding Division, Bangladesh Rice Research Institute, Bangladesh, India;(2) Plant Genetic Manipulation Group. Department of Botany, University of Nottingham, NG7 2RD Nottingham, UK;(3) Present address: Department of Plant Science, Scottish Agricultural College, Ayr KA6 5HW Auchincruive, Scotland |
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| Abstract: | Embryogenic callus initiated from basal segments of micropropagated shoots of Oryza rufipogon were used to initiate cell suspension cultures. After approximately 3 months these cultures were capable of yielding large numbers of protoplasts which underwent sustained division in agarose-solidified medium at a frequency comparable to that observed with Japonica rice protoplasts in previous studies. O. rufipogon plants were reproducibly regenerated from the protoplast-derived callus and are currently being grown to maturity. This is the first report of plant regeneration from protoplasts of a wild species of Oryza.Abbreviations BAP 6-benzyalamino purine - 2,4-D 2,4-dichlorophenoxyacetic acid - MES 2[N-morpholino] ethanesulphonic acid - NAA  -naphthalene acetic acid - PAR photosynthetically active radiation - SCV settled-cell volume |
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