FR167653 suppresses the progression of experimental autoimmune myocarditis |
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Authors: | Maruyama Seitaro Kato Kiminori Kodama Makoto Okura Yuji Hirono Satoru Fuse Koichi Hanawa Haruo Nakagawa Osamu Nakazawa Mikio Miida Takashi Yaoita Eisin Yamamoto Tadashi Inoue Ikuo Aizawa Yoshifusa |
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Institution: | (1) First Department of Internal Medicine, Institute of Nephrology, Niigata University School of Medicine, Niigata, Japan;(2) Department of Medical Technology, Institute of Nephrology, Niigata University School of Medicine, Niigata, Japan;(3) Department of Laboratory Medicine, Institute of Nephrology, Niigata University School of Medicine, Niigata, Japan;(4) Department of Pathology, Institute of Nephrology, Niigata University School of Medicine, Niigata, Japan;(5) The Fourth Department of Internal Medicine, Saitama Medical Schook, Saitama, Japan |
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Abstract: | Experimental autoimmune myocarditis (EAM) induced in rats by injection of cardiac myosin is an animal model of human myocarditis and post-myocarditis dilated cardiomyopathy. It has been reported that proinflammatory cytokines play crucial roles in the induction of EAM and in the progression of myocardial injury in this disease. FR167653 (1-7-(4-fluorophenyl)-1,2,3,4-tetrahydro-8-(4-pyridyl) pyrazolo 5,1-c] 1,2,4] triazin-2-yl]-2-phenylethanedione sulfate monohydrate) as been reported to suppress tumor necrosis factor-alpha (TNF-). We hypothesized that FR167653 would suppress the progression of EAM if TNF- and/or interleukin-1 beta (IL-1) were the culprit cytokines in EAM. To investigate the effects of FR167653 in EAM, FR167653 was given to rats for 4 weeks, immediately after they had been immunized with cardiac myosin. The ratio of heart weight to body weight and the area of inflammatory lesions were less in the FR167653 groups than in the control rats. FR167653 reduced serum sialic acid levels significantly. The control group showed a deterioration in cardiac function. The FR167653 groups had significantly better hemodynamic parameters, including improved left ventricular end-diastolic pressure, central venous pressure, aortic pressure, and positive and negative left ventricular pressure derivatives. mRNA expression of IL-1 in the heart was significantly lower in rats given FR167653. However, mRNA of TNF- was not detected in any groups. Our results suggest that FR167653 suppresses the development of myocarditis by suppression of IL-1. |
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Keywords: | experimental autoimmune myocarditis (EAM) FR167653 IL-1" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0"> TNF-" target="_blank">gif" alt="agr" align="BASELINE" BORDER="0"> ribonuclease protection assay |
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