A rapid one-step purification of NADPH-cytochrome c (P-450) reductase from rat liver microsomes |
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| Authors: | E A Shephard S F Pike B R Rabin I R Phillips |
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| Institution: | 1. Medical and Health Sciences Division, Oak Ridge Associated Universities, Oak Ridge, Tennessee, USA;2. Department of Medicinal Chemistry, School of Medicine, University of North Carolina, Chapel Hill, North Carolina USA |
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| Abstract: | NADPH-cytochrome c (P-450) reductase from liver microsomes of phenobarbital-treated rats has been purified in a single step by affinity chromatography on agarose-hexane-adenosine 2',5'-diphosphate. As determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, enzyme assay, and radioimmunoassay the protein obtained by this single step procedure is as pure as that isolated by multicolumn procedures. |
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| Keywords: | HPLC ether lipids benzoates alkylglycerols alk-1-enylglycerols platelet activating factor |
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