Characterization of WiDr: A human colon carcinoma cell line |
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| Authors: | P. Noguchi R. Wallace J. Johnson E. M. Earley S. O'Brien S. Ferrone M. A. Pellegrino J. Milstien C. Needy W. Browne J. Petricciani |
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| Affiliation: | (1) Division Virology Bureau of Biologics, 20014 Bethesda, Maryland;(2) Lederle Laboratories, 10965 Pearl River, New York;(3) Laboratory of Vinal Carcinogenesis, National Cancer Institute, 20014 Bethesda, Maryland;(4) Department of Molecular Immunology, Scripps Clinic and Research Foundation, La Jolla, California;(5) Division of Pathology, Room 512, Bureau of Biologics, 8800 Rockville Pike, 20014 Bethesda, Maryland |
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| Abstract: | ![]()
Summary We describe the establishment and characterization of WiDr, a cell line derived from a human colon carcinoma. It produces carcinoembryonic antigen in culture, and has a doubling time of 15 hr with plating efficiency of 51%. The HLA antigenic profile and the allozyme genetic signature (composed of eight gene-enzyme systems) of WiDr cells are different from those of HeLa cells. Furthermore, WiDr cells possess three marker chromosomes, again distinct from the HeLa marker chromosomes. Finally, it is highly tumorigenic in four different xenogeneic animal models. Based on these studies, WiDr represents a useful model cell line for tumor cell biology investigations. |
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| Keywords: | human tumor cell line HLA antigens allozyme genetic signature karyology tumorigenicity |
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