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Mitochondrial DNA from lupine: restriction analysis and cloning of fragments coding for tRNA |
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| Authors: | H Augustyniak P Borsuk I Hirschler P P Stepień E Bartnik |
| Affiliation: | 1. Institute of Biochemistry, Adam Mickiewicz University of Poznań, Fredry 10, 61701 Poznań Poland Tel. 224581, ext.186;2. Department of Genetics, University of Warsaw, Al. Ujazdowskie 4, 00478 Warsaw Poland Tel. 294011, ext. 28 |
| Abstract: | Mitochondrial DNA (mtDNA) was isolated from lupine. Restriction analysis was used to estimate its size, which is about 180 kb. A BamHI bank of this mtDNA was constructed using plasmids pBR322 and pBR327 as vectors. Eight clones containing plasmids hybridizing to mitochondrial tRNA (mttRNA) were isolated. Restriction maps of these plasmids were determined. Six of these plasmids hybridized to unique fragments and two to two fragments of very similar size, all obtained by BamHI cleavage of mtDNA. |
| Keywords: | Recombinant DNA plasmid vectors pBR322 and pBR327 plant mttRNA restriction map gene bank EtBr ethidium bromide kb kilobase pairs mtDNA mitochondrial DNA mttRNA mitochondrial tRNA |
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