DNA Polymerase-associated and autonomous vertebrate 3′→5′ exonuleases |
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Authors: | N L Ronzhina T P Kravetskaya V M Krutyakov |
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Institution: | (1) Laboratory of DNA Synthesis, Department of Molecular and Radiation Biophysics, St. Petersburg Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Russia |
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Abstract: | Using methods of gel filtration and ultracentrifugation, cell-free extracts from 12 objects representing the main vertebrate
representatives (bony fish, amphibian, reptiles, birds, mammals, including human) were studied. The enzyme activity of autonomous
3′→5′-exonucleases (AE) has been established to be 25–90% of the total 3′→5′ exonuclease activity of the extracts. A part
of the AE is revealed in a zone of the DNA polymerases of the α-family and can be separated by changing Chromatographic conditions
or by repeated fractionation. The high activity of AE allows suggesting their substantial participation in the replicative
correction of the DNA-poly-merase errors as well as in the postreplicative correction of the heteroduplexes in the vertebrate
DNA. |
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Keywords: | |
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