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Carrageenophyte identification by second-derivative Fourier transform infrared spectroscopy
Authors:P J Cáceres  C A Faúndez  B Matsuhiro  J A Vasquez
Institution:(1) Departamento de Ciencias Químicas, Facultad de Química y Biología, Universidad de Santiago de Chile, casilla 5659, Santiago, 2, Chile;(2) Departamento de Biología Marina, Universidad Católica del Norte, casilla 117, Coquimbo, Chile
Abstract:The second-derivative mode of the Fourier transform I.R. spectra of dried algal material has been applied to distinguish the carrageenans-producingStenogramme interrupta from the isomorphous speciesRhodymenia howeana. Spectra of the tetrasporophyteS. interrupta showed bands assigned to a lambda-carrageenan type polysaccharide, while the gametophytic and cystocarpic plants showed the characteristic absorptions of kappa-and igr-carrageenans. Results were confirmed by hot water extraction of samples of the three nuclear phases ofS. interrupta and characterization of the extracts by chemical analysis.Author for correspondence
Keywords:carrageenans  second-derivative infrared spectroscopy  Stenogramme interrupta
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