| 高速逆流色谱法分离制备花生壳中的黄酮类化合物 |
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| 引用本文： | 牛丹丹,刘彩霞,刘绣华,李明静.高速逆流色谱法分离制备花生壳中的黄酮类化合物[J].天然产物研究与开发,2011,23(1):110-113,158. |
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| 作者姓名： | 牛丹丹 刘彩霞 刘绣华 李明静 |
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| 作者单位： | 1. 河南大学化学化工学院;河南省天然药物与免疫工程重点实验室,开封,475004
2. 河南省天然药物与免疫工程重点实验室,开封,475004 |
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| 基金项目： | 河南省教育厅自然科学研究项目 |
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| 摘 要： | 应用高速逆流色谱法首次从花生壳中分离制备了3种黄酮类化合物。以正己烷-乙酸乙酯-甲醇-水-冰醋酸(5:3:3.5:5:0.25,v/v)为两相溶剂系统,在主机转速800 r/min、流速2 mL/min、检测波长275 nm条件下进行分离制备,纯度用HPLC法测定,各化合物结构经质谱和核磁共振氢谱、碳谱鉴定。结果表明,100 min内从70 mg花生壳粗提物中一步分离制备得到木犀草素11.0 mg,香叶木素2.2 mg,5,7-二羟基色原酮5.2 mg,其纯度均达96.0%以上。利用该方法可以对花生壳中的黄酮类化合物进行快速的分离和纯化。
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| 关 键 词： | 高速逆流色谱 花生壳 木犀草素 香叶木素 5 7-二羟基色原酮 |
Preparative Separation of Flavones from Peanut Hull by High-Speed Counter-Current Chromatography |
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| NIU Dan-dan,LIU Cai-xia,LIU Xiu-hua,LI Ming-jing.Preparative Separation of Flavones from Peanut Hull by High-Speed Counter-Current Chromatography[J].Natural Product Research and Development,2011,23(1):110-113,158. |
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| Authors: | NIU Dan-dan LIU Cai-xia LIU Xiu-hua LI Ming-jing |
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| Institution: | NIU Dan-dan1,2,LIU Cai-xia1,LIU Xiu-hua2,LI Ming-jing1,2 1College of Chemistry and Chemical Engineering,Henan University,Kaifeng 475004,China,2Key Laboratory of Natural Medicine and Immunology Engineering Henan Province |
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| Abstract: | Three flavones were prepared,isolated and purified from peanut hull by high-speed counter-current chromatography (HSCCC) for the first time.A two-phase solvent system composed of n-hexane:ethyl acetate:methanol:water:acetic acid (5:3:3.5:5:0.25,v/v) was used.The obtained fractions were analyzed by HPLC and identified by MS,1H and 13C NMR.Within 100 minute,11.0 mg of luteolin,2.2 mg of diosmetin,and 5.2 mg of 5,7-dihydroxychromone with their purities over 96.0% were obtained from 70 mg crude extract of peanu... |
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| Keywords: | HSCCC peanut hull Luteolin Diosmetin 5 7-dihydroxychromone |
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