玉米19kD醇溶贮藏蛋白基因启动子种子特异性表达控制区段的分析

为研究玉米（Ｚｅａｍａｙｓ　Ｌ．）１９ｋＤ醇溶贮藏蛋白（ｚｅｉｎ）基因启动子种子特异性表达的控制区段，将全长６９４ｂｐ的启动子进行５’端缺失，共得到６个缺失突变体，长度分别为４８８ｂｐ、３７８ｂｐ、３０２ｂｐ、１５２ｂｐ、１２４ｂｐ和８５ｂｐ。将６个片段分别与报告基因ｇｕｓ连接构建成表达载体ｐＤＧＢ系列，经土壤农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍ）介导转化，引入烟草。ＧＵＳ活性检测证明，４８８ｂｐ启动子片段能促使ｇｕｓ基因在种子中特异表达。３７８ｂｐ、３０２ｂｐ、１５２ｂｐ和１２４ｂｐ片段启动子引导的ｇｕｓ基因在烟草根、叶柄、种子中均可表达。

Analysis of the Maize 19 kD Zein Gene Promoter FragmentDriving Seed-specific Expression

A deletion works of a maize 19 kD zein gene promoter in the 5'end was performed and six promoterfragments of different length were obtained. A series of expression vectors was constructed and then transferred into tobacco (Nicotiana tabacum L. ) plants. GUS activity assays indicated that the expression of 488 hp promoter was tissue--specific, for which GUS was active only in transgenic tobacco seeds. The other four fragments containing 378 bp.302 bp.152 bp and 124 bp also have the activity of promoter. They could drive gus gene expressed not only in seeds but also in roots and petioles.