Order of bovine DRB3, DYA,and PRL determined by sperm typing |
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Authors: | Michiel J T van Eijk Ingolf Russ Harris A Lewin |
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Institution: | (1) Laboratory of Immunogenetics, Department of Animal Sciences, University of Illinois at Urbana-Champaign, 206A Plant and Animal Biotechnology Laboratory, 61801 Urbana, Illinois, USA;(2) Department of Animal Genetics, Munich University of Technology, 8050 Freising-Weihenstephan, FRG |
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Abstract: | The order and recombination fractions ( ) between the bovine major histocompatibility complex DRB3, DYA, and prolactin (PRL) genes were determined by typing of 254 sperm from a triply heterozygous bull. A recently developed method, primer extension preamplification (PEP), was used to amplify the bovine sperm genome prior to amplification of specific loci by the polymerase chain reaction (PCR). At least 28 copies of the DRB3, PRL, or DYA gene were obtained from 50 cycles of PEP. For sperm typing, alleles of each locus were discriminated by restriction endonuclease cleavage of PCR products and polyacrylamide gel electrophoresis of the restriction fragments. The most likely gene order is PRL-DRB3-DYA, with =0.025 (±0.012) and =0.150 (±0.024), respectively. The odds are 128:1 in favor of this order in comparison with the second most likely order DRB3-PRL-DYA. Our results demonstrate the power of sperm typing in concert with PEP for multilocus gene mapping. |
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