| 一种新型抗菌肽Cecropin B真核表达载体的构建 |
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| 引用本文： | 胡仲秋,樊钰虎,冯瑄,杨晓燕,边六交.一种新型抗菌肽Cecropin B真核表达载体的构建[J].生物技术通讯,2005,16(3):268-270. |
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| 作者姓名： | 胡仲秋 樊钰虎 冯瑄 杨晓燕 边六交 |
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| 作者单位： | 西北大学,基因工程药物研究开发中心,陕西,西安,710069 |
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| 基金项目： | 陕西省科技攻关项目(2003K10-G58) |
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| 摘 要： | 通过重叠区扩增法人工设计和合成一种新型抗菌肽Cecropin B基因，按正确的阅读框架定向克隆至巴斯德毕赤酵母的高效表达载体pPIC9K上。经PCR鉴定及序列分析，所转化的大肠杆菌DH5ct菌落中含有插入Cecropin B基因的重组质粒pPIC9K—CB，表明成功构建了新型抗菌肽Cecropin B表达载体pPIC9K—CB。
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| 关 键 词： | Cecropin 抗菌肽 真核表达载体 构建 巴斯德毕赤酵母 高效表达载体 PCR鉴定 人工设计 定向克隆 序列分析 大肠杆菌 重组质粒 B基因 重叠区 CB 菌落 |
| 文章编号： | 1009-0002(2005)03-0268-03 |
| 修稿时间： | 2004年12月27 |
Construction of the Eukaryotic Recombinant Expression Plasmid for a Novel Cecropin B |
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| HU Zhong-qiu,FAN Yu-hu,FENG Xuan,YANG Xiao-yan,BIAN Liu-jiao.Construction of the Eukaryotic Recombinant Expression Plasmid for a Novel Cecropin B[J].Letters in Biotechnology,2005,16(3):268-270. |
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| Authors: | HU Zhong-qiu FAN Yu-hu FENG Xuan YANG Xiao-yan BIAN Liu-jiao |
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| Institution: | HU Zhong-qiu,FAN Yu-hu,FENG Xuan,YANG Xiao-yan,BIAN Liu-jiao Center of Gene-engineering,Northwest University,Xi'an 710069,China |
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| Abstract: | A novel antibacterial peptide cecropin B gene was designed and artificially synthesized through using gene splicing by overlap extension (gene SOEing) method. After this cecropin B gene was subcloned into plasmid pPIC9K, a novel recombinant expression plasmid pPIC9K-CB, which can be efficiently expressed in Pichia pastoris, can be obtained. By using PCR and sequence analyses, it can be proved that all the tested clones contain this novel cecropin B gene, and the novel cecropin B expression plasmid pPIC9K-CB is successfully constructed. |
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| Keywords: | cecropin B splicing by overlap extension recombinant expression plasmid eukaryotic expression |
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