Development and validation of a liquid chromatographic method for Casiopeina IIgly in rat plasma |
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Authors: | Reyes Leobardo Fuentes-Noriega Inés Ruiz-Ramìrez Lena Macías Lucía |
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Institution: | Departamento de Farmacia, Universidad Nacional Autónoma de México, México DF 04510, Mexico. |
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Abstract: | A sensitive and specific liquid chromatographic method using solid-phase extraction with Sep-pak cartridges has been developed for the determination of Casiopeina IIgly and validated over the linear range 2.5-50 microg/ml in rat plasma. The analysis was performed on a Symetry C(18) (5 microm) column with a Phenomenex C(18) precolumn. The mobile phase was methanol-water (58:42, v/v). The column effluent was monitored at 273 nm. The results showed that the assay is sensitive at 2.5 microg/ml. Maximum intra-day coefficient of variation was 11.47%. The recovery based upon addition of internal standard to rat plasma was 80.98%. The method was used to perform preclinical pharmacokinetic studies in rat plasma and was found to be satisfactory. |
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