Optimality assessment in the enzyme-linked immunosorbent assay (ELISA) |
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Authors: | K F Karpinski |
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Institution: | Health Protection Branch, Health and Welfare Canada, Ottawa, Ontario. |
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Abstract: | An optimality criterion is proposed for evaluating the precision of alternative designs in the enzyme-linked immunosorbent assay. Assay profiles are represented as four-parameter logistic functions with parameter estimation based on either a weighted nonlinear regression or a simple nonlinear regression after a logarithmic transformation. Assay design changes are characterized in terms of their effects on parameters in the four-parameter logistic model. General optimality results are derived for the variance of relative potency estimates in routine assay applications. |
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