An efficient procedure for embryogenic callus induction and double haploid plant regeneration through anther culture of Thai aromatic rice (<Emphasis Type="Italic">Oryza sativa</Emphasis> L. subsp. <Emphasis Type="Italic">indica</Emphasis>) |
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Authors: | Suriyan Cha-um Bootsaya Srianan Aussanee Pichakum Chalermpol Kirdmanee |
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Institution: | (1) National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Paholyothin Rd, Klong Luang, Pathumthani, 12120, Thailand;(2) Department of Plant Science, Faculty of Science, Mahidol University, Rama VI Rd, Payathai, Bangkok, 10400, Thailand |
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Abstract: | Anther culture is a biotechnology technique that can be used for the production of pure lines. The aims of this investigation
were to induce embryogenic callus from major and minor culms of Thai aromatic rice cultivars and to subsequently regenerate
double-haploid green plantlets by the application of exogenous polyamines. Embryogenic callus derived from anther culture
was successfully induced in varieties KDML105, Homjan (HJ), and Pathumthani 1 (PT1). Production of embryogenic callus from
anthers collected from the major culms was greater than those collected from the minor culms, especially in cultivar HJ. Plantlet
regeneration in the three rice cultivars was observed from embryogenic callus and was highest, at 12.1%, from variety HJ treated
with 0.5 mM spermidine. Plantlet regeneration from anther-derived embryogenic callus was dependent on the plant genotype,
the types of exogenous polyamines, and the interactions of these factors. The percentage of haploid plantlets regenerated
in PT1, KDML105, and HJ were 68.1%, 70.7%, and 78.5%, respectively. Only haploid plantlets were treated with colchicine for
double-haploid production. This investigation has increased the knowledge of both embryogenic callus induction and plantlet
regeneration in aromatic rice and has lead to the development of a pure, double-haploid line for the use in rice breeding
programs in Thailand. |
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Keywords: | Aromatic rice Culms Donor plant maturity Polyamines Pure line Spermidine |
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