Terminal fragments of herpes simplex virus DNA produced by restriction endonuclease. |
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Authors: | W L Steinhart R H Grafstrom C W Hill |
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Institution: | Department of Biology Bowdoin College Brunswick, ME 04011 USA;Department of Biological Chemistry The Milton S. Hershey Medical Center The Pennsylvania State University Hershey, PA 17033 USA |
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Abstract: | Digestion of HSV-1 DNA with λ 5′-exonuclease prior to digesting the DNA with the I restriction endonuclease specifically affects two of the fragments normally obtained after restriction endonuclease digestion. Therefore these two fragments contain the sequences which occur at the termini of HSV-1 DNA. One of the fragments affected is a “minor” fragment which is always present in less than molar yield. The possible relationship between the occurrence of minor I fragments and the partial refractoriness of HSV-1 DNA to λ 5′-exonuclease digestion is discussed. |
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