Immunoassay of the Neuronal and Neuroendocrine Marker PGP 9.5 in Human Tissues |
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Authors: | Jane M Bradbury R J Thompson |
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Institution: | Department of Clinical Biochemistry, School of Clinical Medicine, Addenbrooke's Hospital, Hills Road, Cambridge, U.K. |
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Abstract: | An inhibitory, coated-well immunoassay for the neurone-specific protein PGP 9.5 has been devised and used to measure the concentrations of the protein in human tissues. Concentrations of PGP 9.5 between 40 ng/ml and 10 micrograms/ml could be measured using this assay. In brain PGP 9.5 was present at 100.58 +/- 16.18 micrograms/mg protein. Of the other organs examined only kidney and testis showed significant concentrations of PGP 9.5 (3.97 +/- 0.87 microgram/mg protein and 3.25 +/- 0.36 microgram/mg protein, respectively). All other organs contained less than 2% of the brain level. The tissue levels determined by coated-well immunoassay confirmed the tissue specificity of PGP 9.5 originally determined by high-resolution two-dimensional gel electrophoresis. |
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Keywords: | Neurone-specific protein PGP 9 5 APUD cells Immunoassay Human tissues |
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