The effect of zinc stress combined with high irradiance stress on membrane damage and antioxidative response in bean seedlings |
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| Institution: | 1. Global Wheat Program, International Maize and Wheat Improvement Center (CIMMYT), Apdo Postal 6-641, Mexico DF, Mexico;2. Campo Experimental Valle de Mexico INIFAP, Apdo. Postal 10, 56230 Chapingo, Edo de Mexico, Mexico;3. Global Conservation Agriculture Program, International Maize and Wheat Improvement Center (CIMMYT), Apdo Postal 6-641, Mexico DF, Mexico;1. Department of Biology, Faculty of Basic Science, University of Mazandaran, Babolsar, Iran;2. Laboratory of Plant Physiology, Groningen Institute for Evolutionary Life Sciences, University of Groningen, P.O. Box 11103, 9700 CC Groningen, the Netherlands;3. DynaMo Center, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark;1. Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, Xiamen University, Xiamen 361005, China;2. State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China;3. Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;1. State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling 712100, Shaanxi, China;2. Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Ministry of Agriculture / College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, Shaanxi, China;3. Department of Renewable Resources, University of Alberta, Edmonton, Alberta, Canada |
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| Abstract: | Zinc (Zn) is a necessary element for plants, but excess Zn can be detrimental. The effect of Zn and high irradiance (HI) stress on the growth, lipid peroxidation (MDA), membrane permeability (EC), hydrogen peroxide (H2O2) accumulation, non-enzymatic antioxidants like proline accumulation and ascorbic acid (AsA) and the activities of major antioxidant enzymes (superoxide dismutase, SOD; peroxidase, POX; polyphenol oxidase, PPO) of bean leaves were investigated under controlled growth conditions. The root length was not reduced at excess Zn level. Application of Zn significantly increased Zn concentration in the leaves of bean plants. Under Zn and HI stress, the Zn-deficient and Zn-excess conditions significantly increased the EC, MDA and H2O2 content of excised leaves of bean. The SOD activity was found to be increased significantly in both Zn-deficiency and Zn-excess leaves under Zn and HI stress. Under both Zn and HI stress conditions, the antioxidant enzyme activities; POX, PPO and the non-enzymatic antioxidants, AsA and proline accumulation were found to be significantly increased in the Zn-excess leaves which showed that the bean plant had the ability to tolerate the excess level of Zn and HI stress. A significant increase in MDA, H2O2, and EC with a simultaneous decrease in the antioxidant enzyme activities under Zn-deficiency compared to Zn-sufficient condition shows the inefficiency of the bean plant in response to Zn deficiency. To the best of our knowledge, this is the first report on the effect of Zn stress combined with HI stress in bean plant. |
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