Complete nucleotide sequence of pAL5000, a plasmid from Mycobacterium fortuitum |
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| Authors: | J Rauzier J Moniz-Pereira B Gicquel-Sanzey |
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| Affiliation: | Unité de Génie Microbiologique, Institut Pasteur, Paris, France. |
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| Abstract: | We have determined the complete nucleotide sequence of pAL5000, a plasmid from Mycobacterium fortuitum; the plasmid contains 4837 bp with 65% G + C. Five open reading frames (ORF1 to ORF5) have been identified. A number of sequences corresponding to palindromes, repeats, a helix-turn-helix motif, a signal sequence and repetitive amino acid motifs can be identified. This sequence should facilitate the construction of vectors based on pAL5000 for transfer and expression studies in mycobacteria. |
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