Phosphatidylethanolamine Deficiency Impairs Escherichia coli Adhesion by Downregulating Lipopolysaccharide Synthesis,Which is Reversible by High Galactose/Lactose Cultivation |
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| Authors: | Chuan Yu Ming Li Yanan Sun Xingguo Wang |
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| Institution: | 1. College of Life Sciences, Nanchang University, Nanchang, Jiangxi, P.R. China;2. Nanoscale Science and Technology Laboratory, Institute for Advanced Study, Nanchang University, Nanchang, Jiangxi, P.R. China;3. Faculty of Life Sciences, Hubei University, Wuchang, Hubei, P.R. China |
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| Abstract: | As the initiation step of bacterial infection or biofouling, bacterial adhesion on cells or substrates is generally an optimal target for antibacterial design. Phosphatidylethanolamine (PE) is the principal phospholipid in bacteria, and its function in bacterial adhesion remains unclear. In this study, four E. coli strains including two PE-deficient mutants (PE?PC? and PE?PC+?strains) and two PE-containing wild-type controls (PE?+?PC? strains) were recruited to investigate the influence of PE deficiency on bacterial adhesion. We found that PE deficiency could impair E. coli adhesion on macrophages (human THP-1-derived and mouse RAW264.7 macrophages) or glass coverslips by downregulating lipopolysaccharide (LPS) biosynthesis, which could be reversible by high galactose/lactose but not glucose cultivation. The data imply that PE play important role in bacterial adhesion probably via affecting LPS biosynthesis and suggest that targeting PE biosynthesis is also a potential antibacterial strategy. |
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| Keywords: | Escherichia coli phosphatidylethanolamine lipopolysaccharide bacterial adhesion macrophage high sugar cultivation |
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